The PlasmaKnife testing data on this page falls into several categories. First there is data that has been generated during IonField’s SBIR contract with the National Center for the Advancing Translational Science (NCATS). NCATS is the newest of the 27 Institutes and Centers (IC) of the NIH.
The second type of data is being generated in conjunction with potential beta test partner companies. The process starts with a test of each type of plate being processed with a PlasmaKnife. This test is to document what effect, if any, the PlasmaKnife will have on a microplate . The graph to the upper right shows the difference between a luciferase assay in a PlasmaKnife processed 1536 plate and the same assay run in a new plate. The total count difference between the plates was 179 and the CVs and other statistical data were virtually identical.
The next two graphs to the right show data from label free microplate testing. Just to the right, a 1536 plate was cleaned once and 25 times. Those plates are compared to a new plate. The lower graph shows results with 384 plates cleaned once and 5 times; again compared to a new plate. “Compound 1” is a placeholder for the real compound names.
Plate types currently being tested include:
Real Time PCR
The PlasmaKnife process does appear, based on preliminary data seen to the left, to slightly alter the number of cycles required for real time PCR. We are setting up testing to determine if minor lengthening of the cycle timing or increase in the target temperature (by a 1 or 2 10ths of a degree) will offset this effect. The calculations from the rtPCR results are identical using new or plasma treated plates.
Acoustic Source Plates
Acoustic Source Plates have been tested at multiple companies and there is no alteration to the plate performance by any measurement. Both accounts are planning to PlasmaKnife treat their source plates and then put the same compound back into the same well. Bar codes do not need to be changed and down time is limited to the time needed for PlasmaKnife processing and the plates to be refilled. Both accounts expect to save between 1/4 and 1/2 million dollars per year just on 1536 source plates.
& High Content 1536 WiScan
IonField is working with a number of companies to optimize the plasma treatment of Ionchannel Plates. To the left is representative data from one of those companies. The plasma treated plate data is similar to data from other plates they run. And their comment is that they look good based on their experience.
The development team at IonField works closely with many leading researchers to insure our system delivers exceptional cleaning for all types of microplates. Every facet of the system is rigorously tested to insure optimum effectiveness and long term reliability. We will continue to optimize system performance and are building into the PlasmaKnife the operational settings to meet the unique parameters needed for all plate types.
At the bottom of this page are two scans comparing 1536 WiScan results of new versus plasma treated microplates in green and blue channels.
Contracts are now being negotiated for a limited number of beta program units with delivery in 5 to 6 months. The PlasmaKnife will be available for sale early Q3 2016.